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Proceedings Paper

Picosecond Fluorescence Studies Of Polypeptide Dynamics
Author(s): Lin X.-Q. Chen; Jacob W Petrich; Angelo Perico; Graham R Fleming
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Paper Abstract

Fluorescence anisotropies for single tryptophan-containing polypeptide hormones ACTH and glucagon, and a series of their fragments are studied. The data are discussed in the context of the theory of Perico and Guenza (J. Chem. Phys. 84, 510 (1986)) and a persistence length of about 7 to 10 residues obtained for the mobilities in the two hormones. Theory is able to account for chain length and probe location effects very well. The observed discrepencies between calculated and measured anisotropy decays very likely arise from approximations made in the calculation and from the limited time resolution of the experiments.

Paper Details

Date Published: 24 June 1988
PDF: 7 pages
Proc. SPIE 0909, Time-Resolved Laser Spectroscopy in Biochemistry, (24 June 1988); doi: 10.1117/12.945391
Show Author Affiliations
Lin X.-Q. Chen, The University of Chicago (United States)
Jacob W Petrich, The University of Chicago (United States)
Angelo Perico, Instituto di Chimica Industriale (Italy)
Graham R Fleming, The University of Chicago (United States)

Published in SPIE Proceedings Vol. 0909:
Time-Resolved Laser Spectroscopy in Biochemistry
Joseph R. Lakowicz, Editor(s)

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