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Proceedings Paper

Fluorescence Studies Of Bilayers And Proteins: Critical Behavior And Genetic Engineering
Author(s): Bruce Hudson; Anthony Ruggiero; Danni Harris; lain Johnson; Xiu -mei Dou; Thomas Novet; Lawrence McIntosh; Cynthia Phillips; Theresa Nester
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Paper Abstract

Fluorescence decay and anisotropy studies of parinaric acid in phospholipid bilayers have demonstrated the presence of density fluctuations exhibiting critical behavior in these nominally single phase structures. The anisotropy behavior is used to extract the second and fourth rank order parameters <P2> and <P4> for comparison with models of acyl chain order. A recent study of parinaric acid in hexagonal urea inclusion complexes has demonstrated that the strongly allowed transition is polarized at an angle with respect to the chain axis. The implications of this for the interpretation of anisotropy experiments is discussed. The lysozyme from bacteriophage aq has been engineered in modified forms containing only one tryptophan residue and with substitutions near the buried tryptophan residue 138. Simple changes in the structure appear to result in large changes in the dynamics of this residue. These observations are compared with the results of molecular dynamics computations.

Paper Details

Date Published: 24 June 1988
PDF: 8 pages
Proc. SPIE 0909, Time-Resolved Laser Spectroscopy in Biochemistry, (24 June 1988); doi: 10.1117/12.945375
Show Author Affiliations
Bruce Hudson, University of Oregon (United States)
Anthony Ruggiero, University of Oregon (United States)
Danni Harris, University of Oregon (United States)
lain Johnson, University of Oregon (United States)
Xiu -mei Dou, University of Oregon (United States)
Thomas Novet, University of Oregon (United States)
Lawrence McIntosh, University of Oregon (United States)
Cynthia Phillips, University of Oregon (United States)
Theresa Nester, University of Oregon (United States)

Published in SPIE Proceedings Vol. 0909:
Time-Resolved Laser Spectroscopy in Biochemistry
Joseph R. Lakowicz, Editor(s)

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