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Proceedings Paper

Synchrotron Radiation As An Excitation Source In Time-Domain And Frequency-Domain Fluorometry
Author(s): William R Laws; John Clark Sutherland
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Paper Abstract

Synchrotron radiation is an excellent excitation source for both the time-domain (pulse) and the frequency-domain (phase/modulation) methods of time-resolved fluorescence spectroscopy. All possible wavelengths are available to excite fluorescent biological probes since synchrotron radiation consists of an intense continuum of energies from the IR to X-rays. The pulsed nature of synchrotron radiation permits time-correlated single photon-counting techniques to collect a histogram of the probability of the decay of the excited state. The harmonics of the pulsed exciting light also provide a wide range of modulation frequencies that permit the frequency-domain method to match the current resolution for time-domain measurements.

Paper Details

Date Published: 24 June 1988
PDF: 6 pages
Proc. SPIE 0909, Time-Resolved Laser Spectroscopy in Biochemistry, (24 June 1988); doi: 10.1117/12.945366
Show Author Affiliations
William R Laws, Mount Sinai School of Medicine (United States)
John Clark Sutherland, Brookhaven National Laboratory (United States)

Published in SPIE Proceedings Vol. 0909:
Time-Resolved Laser Spectroscopy in Biochemistry
Joseph R. Lakowicz, Editor(s)

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