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Proceedings Paper

Gigahertz Frequency-Domain Fluorometry: Applications To Picosecond Processes And Future Developments
Author(s): Joseph R Lakowicz; Gabor Laczko; Ignacy Gryczynski; Henryk Cherek; Wieslaw Wiczk
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Paper Abstract

We describe our frequency-domain fluorometer which allows phase angle and demodulation measurements from 8 MHz to 2 GHz. This instrument using the harmonic content of a 7.59 MHz train of 5 ps pulses as the modulated excitation source. The detector is a microchannel plate PMT (R1564U), whose bandwidth extends to 2 GHz. Using this instrument we examined rapid rotational motions of indole in low viscosity solvents. To date, the shortest correlation time we have determined was 7 ps, for indole in methanol at 80°C. For indole in cyclohexane at 20°C we were able to resolve two rotational correlation times of 17 and 73 ps. It now appears that the frequency range can be extended to 8 GHz, so still faster processes should be observable.

Paper Details

Date Published: 24 June 1988
PDF: 8 pages
Proc. SPIE 0909, Time-Resolved Laser Spectroscopy in Biochemistry, (24 June 1988); doi: 10.1117/12.945364
Show Author Affiliations
Joseph R Lakowicz, University of Maryland at Baltimore (United States)
Gabor Laczko, University of Maryland at Baltimore (United States)
Ignacy Gryczynski, University of Maryland at Baltimore (United States)
Henryk Cherek, University of Maryland at Baltimore (United States)
Wieslaw Wiczk, University of Maryland at Baltimore (United States)

Published in SPIE Proceedings Vol. 0909:
Time-Resolved Laser Spectroscopy in Biochemistry
Joseph R. Lakowicz, Editor(s)

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