The use of a laser Doppler microscope, developed to measure flow and/or diffusion in living cells, is described. It analyses Doppler shifts given to the frequency of laser light scattered at a known angle from particles moving in a volume of less than 200 cubic micrometres. This scattering volume is defined in the specimen by an aperture in an image-plane and can be placed within a single cell. Specimens can be viewed and recorded continuously by video-enhanced differential interference contrast microscopy during experiments so that, independently of laser-Doppler measurements, sizes of individual particles in images may be measured and their displacements timed with the aid of a video-micrometer. This paper describes how ways are now needed to constrain and improve computation and analysis of the laser Doppler measurements by incorporating information from the images.

Date Published: 10 September 1987
PDF: 5 pages
Proc. SPIE 0808, Inverse Problems in Optics, (10 September 1987); doi: 10.1117/12.941473

Published in SPIE Proceedings Vol. 0808:
Inverse Problems in Optics
Edward Roy Pike M.D., Editor(s)