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Proceedings Paper

Porous silicon photonic crystals for detection of infections
Author(s): B. Gupta; B. Guan; P. J. Reece; J. J. Gooding
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Paper Abstract

In this paper we demonstrate the possibility of modifying porous silicon (PSi) particles with surface chemistry and immobilizing a biopolymer, gelatin for the detection of protease enzymes in solution. A rugate filter, a one-dimensional photonic crystal, is fabricated that exhibits a high-reflectivity optical resonance that is sensitive to small changes in the refractive index. To immobilize gelatin in the pores of the particles, the hydrogen-terminated silicon surface was first modified with an alkyne, 1,8-nonadiyne via hydrosilylation to protect the silicon surfaces from oxidation. This modification allows for further functionality to be added such as the coupling of gelatin. Exposure of the gelatin modified particles to the protease subtilisin in solution causes a change in the refractive index, resulting in a shift of the resonance to shorter wavelengths, indicating cleavage of organic material within the pores. The ability to monitor the spectroscopic properties of microparticles, and shifts in the optical signature due to changes in the refractive index of the material within the pore space, is demonstrated.

Paper Details

Date Published: 10 October 2012
PDF: 8 pages
Proc. SPIE 8460, Biosensing and Nanomedicine V, 84600Z (10 October 2012); doi: 10.1117/12.940757
Show Author Affiliations
B. Gupta, The Univ. of New South Wales (Australia)
B. Guan, The Univ. of New South Wales (Australia)
P. J. Reece, The Univ. of New South Wales (Australia)
J. J. Gooding, The Univ. of New South Wales (Australia)

Published in SPIE Proceedings Vol. 8460:
Biosensing and Nanomedicine V
Hooman Mohseni; Massoud H. Agahi; Manijeh Razeghi, Editor(s)

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