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Proceedings Paper

Comparison Of Image Averaging Methods For Structure Determination In Molecular Biology
Author(s): Olaf Kubler; Dietmar Pum
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Paper Abstract

Structure determination in molecular biology with the aid of the electron microscope is made difficult by the extreme susceptibility of biological objects to ionizing radiation. Molecular structures may be assessed after averaging over many low dose images of like preparations. Signal averaging is facilitated when periodic structures such as crystalline membrane components are investigated. Fourier techniques akin to methods of X-ray crystallography or matched filter techniques may be used to obtain the average structure. The alternative methods, their reliability and their usage were investigated on computer simulations of electron microscopical images.

Paper Details

Date Published: 9 January 1984
PDF: 9 pages
Proc. SPIE 0432, Applications of Digital Image Processing VI, (9 January 1984); doi: 10.1117/12.936635
Show Author Affiliations
Olaf Kubler, Institut fur Kommunikationstechnik (Switzerland)
Dietmar Pum, University of Agriculture (Austria)

Published in SPIE Proceedings Vol. 0432:
Applications of Digital Image Processing VI
Andrew G. Tescher, Editor(s)

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