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Proceedings Paper

Wavelength Prograimable Spectrophotometer For Individual Plant Studies
Author(s): E J Brach; J Simmonds; P Poirier
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Paper Abstract

Action spectra for a number of light-mediated physiological processes, (e.g. germination, flowering, elongation) indicated that the effective wavelength for induction was between 600-700 nm and for supression was between 700-760 nm, with maxima at 660 nm and 730 nm respectively (see Smith 1975 for review). These studies predicted the existence of the photoreversible pigment phytochrome (P) existing in two forms, interconvertible by red and far-red light. The photo-equilibrium of the red absorbing (Pr) and far-red absorbing (Pfr) forms is determined by the proportions of red and far-red light available. Most of the infornation cooes from studies on dark grown plants using narrow band or uonochromatic light and until recently very little work has been done on the role of phytochrome in the natural environment. Because changes in the distribution of this physiologically active light in nature will result in an altered photo-equilibrium of the two forms of phytochrome, a new quantity c (zeta) was defined, as the ratio of the quantum flux at 660 ni to the quantum flux at 730 nm (Holmes and McCartney 1976, Monteith 1976). This relationship of zeta to the photochrome photoequilibrium (% Pfr) was determined for a series of natural and artificial light sources (Smith and Holmes 1977). owever, radiation of shorter wavelengths also has an infuence on plant development through its action on phytochrome (Parker et al 1946, Bertsch 1963). The absorption spectra of the two forms of phytochrome show, in addition to the vajor absorption bands in the red and far-red regions, minor bands in the blue and near uv (Hendricks 1962, Siegelman and Fuer 1964). Also photochrome does undergo light-induced absorbance changes 'in vitro' in the blue region of the spectrum (Everett and Briggs 1970). A more accurate estimate of photochrome photoequilibria would

Paper Details

Date Published: 7 November 1983
PDF: 7 pages
Proc. SPIE 0380, Los Alamos Conf on Optics '83, (7 November 1983); doi: 10.1117/12.934743
Show Author Affiliations
E J Brach, Engineering and Statistical Research Institute (Canada)
J Simmonds, Ottawa Research Station (Canada)
P Poirier, Engineering and Statistical Research Institute (Canada)

Published in SPIE Proceedings Vol. 0380:
Los Alamos Conf on Optics '83
Robert S. McDowell; Susanne C. Stotlar, Editor(s)

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