Share Email Print

Proceedings Paper

Video Imaging System For Microscopy: Technical Aspects
Author(s): H. Callisen; M. Pincu; A. Norman
Format Member Price Non-Member Price
PDF $17.00 $21.00

Paper Abstract

In order to quantitatively study cellular and nuclear properties, we have assembled a low cost video/microscope densitometric system. The system consists of a conventional light microscope coupled to a low light level TV camera whose output is sequentially processed by two microcomputers, one for image acquisition and one for data analysis. The system allows us to a) acquire and store microscope images, b) make spatial measurements such as size and shape, and c) make grayness measurements needed to compute optical density and texture descriptors. Despite the inherent limitations of a video micrometry system (e.g. poor signal-to-noise ratio, automatic gain compensations, etc.) we have been able to collect reproducible data with satisfactory accuracies.

Paper Details

Date Published: 1 November 1982
PDF: 4 pages
Proc. SPIE 0372, Physics and Engineering in Medical Imaging, (1 November 1982); doi: 10.1117/12.934492
Show Author Affiliations
H. Callisen, University of California (United States)
M. Pincu, University of California (United States)
A. Norman, University of California (United States)

Published in SPIE Proceedings Vol. 0372:
Physics and Engineering in Medical Imaging
Orhan Nalcioglu, Editor(s)

© SPIE. Terms of Use
Back to Top
Sign in to read the full article
Create a free SPIE account to get access to
premium articles and original research
Forgot your username?