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Proceedings Paper

Electrical detection of specific versus non-specific binding events in breast cancer cells
Author(s): Benjamin C. King; Michael Clark; Thomas Burkhead; Palaniappan Sethu; Shesh Rai; Goetz Kloecker; Balaji Panchapakesan
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Paper Abstract

Detection of circulating tumor cells (CTCs) from patient blood samples offers a desirable alternative to invasive tissue biopsies for screening of malignant carcinomas. A rigorous CTC detection method must identify CTCs from millions of other formed elements in blood and distinguish them from healthy tissue cells also present in the blood. CTCs are known to overexpress surface receptors, many of which aid them in invading other tissue, and these provide an avenue for their detection. We have developed carbon nanotube (CNT) thin film devices to specifically detect these receptors in intact cells. The CNT sidewalls are functionalized with antibodies specific to Epithelial Cell Adhesion Molecule (EpCAM), a marker overexpressed by breast and other carcinomas. Specific binding of EpCAM to anti-EpCAM antibodies causes a change in the local charge environment of the CNT surface which produces a characteristic electrical signal. Two cell lines were tested in the device: MCF7, a mammary adenocarcinoma line which overexpresses EpCAM, and MCF10A, a non-tumorigenic mammary epithelial line which does not. Introduction of MCF7s caused significant changes in the electrical conductance of the devices due to specific binding and associated charge environment change near the CNT sidewalls. Introduction of MCF10A displays a different profile due to purely nonspecific interactions. The profile of specific vs. nonspecific interaction signatures using carbon based devices will guide development of this diagnostic tool towards clinical sample volumes with wide variety of markers.

Paper Details

Date Published: 10 October 2012
PDF: 12 pages
Proc. SPIE 8460, Biosensing and Nanomedicine V, 84600S (10 October 2012); doi: 10.1117/12.929875
Show Author Affiliations
Benjamin C. King, Univ. of Louisville (United States)
Michael Clark, Univ. of Louisville (United States)
Thomas Burkhead, Univ. of Louisville (United States)
Palaniappan Sethu, Univ. of Louisville (United States)
Shesh Rai, James Graham Brown Cancer Ctr. (United States)
Goetz Kloecker, James Graham Brown Cancer Ctr. (United States)
Balaji Panchapakesan, Univ. of Louisville (United States)

Published in SPIE Proceedings Vol. 8460:
Biosensing and Nanomedicine V
Hooman Mohseni; Massoud H. Agahi; Manijeh Razeghi, Editor(s)

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