Share Email Print

Proceedings Paper

Combined micro computed tomography and histology study of bone augmentation and distraction osteogenesis
Author(s): Bernd Ilgenstein; Hans Deyhle; Claude Jaquiery; Christoph Kunz; Anja Stalder; Stefan Stübinger; Gernot Jundt; Felix Beckmann; Bert Müller; Simone Elke Hieber
Format Member Price Non-Member Price
PDF $17.00 $21.00

Paper Abstract

Bone augmentation is a vital part of surgical interventions of the oral and maxillofacial area including dental implantology. Prior to implant placement, sufficient bone volume is needed to reduce the risk of peri-implantitis. While augmentation using harvested autologous bone is still considered as gold standard, many surgeons prefer bone substitutes to reduce operation time and to avoid donor site morbidity. To assess the osteogenic efficacy of commercially available augmentation materials we analyzed drill cores extracted before implant insertion. In younger patients, distraction osteogenesis is successfully applied to correct craniofacial deformities through targeted bone formation. To study the influence of mesenchymal stem cells on bone regeneration during distraction osteogenesis, human mesenchymal stem cells were injected into the distraction gap of nude rat mandibles immediately after osteotomy. The distraction was performed over eleven days to reach a distraction gap of 6 mm. Both the rat mandibles and the drill cores were scanned using synchrotron radiation-based micro computed tomography. The three-dimensional data were manually registered and compared with corresponding two-dimensional histological sections to assess bone regeneration and its morphology. The analysis of the rat mandibles indicates that bone formation is enhanced by mesenchymal stem cells injected before distraction. The bone substitutes yielded a wide range of bone volume and degree of resorption. The volume fraction of the newly formed bone was determined to 34.4% in the computed tomography dataset for the augmentation material Geistlich Bio-Oss®. The combination of computed tomography and histology allowed a complementary assessment for both bone augmentation and distraction osteogenesis.

Paper Details

Date Published: 17 October 2012
PDF: 13 pages
Proc. SPIE 8506, Developments in X-Ray Tomography VIII, 85060M (17 October 2012); doi: 10.1117/12.929616
Show Author Affiliations
Bernd Ilgenstein, Basel Univ. Hospital (Switzerland)
Hans Deyhle, Basel Univ. Hospital (Switzerland)
Claude Jaquiery, Basel Univ. Hospital (Switzerland)
Christoph Kunz, Basel Univ. Hospital (Switzerland)
Anja Stalder, Basel Univ. Hospital (Switzerland)
Stefan Stübinger, Univ. of Zurich (Switzerland)
Gernot Jundt, Basel Univ. Hospital (Switzerland)
Felix Beckmann, Helmholtz-Zentrum Geesthacht (Germany)
Bert Müller, Basel Univ. Hospital (Switzerland)
Simone Elke Hieber, Basel Univ. Hospital (Switzerland)

Published in SPIE Proceedings Vol. 8506:
Developments in X-Ray Tomography VIII
Stuart R. Stock, Editor(s)

© SPIE. Terms of Use
Back to Top
Sign in to read the full article
Create a free SPIE account to get access to
premium articles and original research
Forgot your username?