
Proceedings Paper
Induction of sustained glycolytic oscillations in single yeast cells using microfluidics and optical tweezersFormat | Member Price | Non-Member Price |
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Paper Abstract
Yeast glycolytic oscillations have been studied since the 1950s in cell free extracts and in intact cells. Until recently,
sustained oscillations have only been observed in intact cells at the population level. The aim of this study was to
investigate sustained glycolytic oscillations in single cells.
Optical tweezers were used to position yeast cells in arrays with variable cell density in the junction of a microfluidic
flow chamber. The microfluidic flow chambers were fabricated using soft lithography and the flow rates in the different
inlet channels were individually controlled by syringe pumps. Due to the low Reynolds number, the solutions mixed by
diffusion only. The environment in the junction of the chamber could thus be controlled by changing the flow rates in the
inlet channels, with a complete change of environment within 2 s. The optimum position of the cell array was determined
by simulations, to ensure complete coverage of the intended solution without any concentration gradients over the cell
array. Using a DAPI filter set, the NADH auto fluorescence could be monitored in up to 100 cells simultaneously.
Sustained oscillations were successfully induced in individual, isolated cells within specific flow rates and
concentrations of glucose and cyanide. By changing the flow rates without changing the surrounding solution, it was
found that the cell behavior was dependent on the concentration of chemicals in the medium rather than the flow rates in
the range tested. Furthermore, by packing cells tightly, cell-to-cell interaction and synchronization could be studied.
Paper Details
Date Published: 10 October 2012
PDF: 7 pages
Proc. SPIE 8458, Optical Trapping and Optical Micromanipulation IX, 84580Y (10 October 2012); doi: 10.1117/12.928919
Published in SPIE Proceedings Vol. 8458:
Optical Trapping and Optical Micromanipulation IX
Kishan Dholakia; Gabriel C. Spalding, Editor(s)
PDF: 7 pages
Proc. SPIE 8458, Optical Trapping and Optical Micromanipulation IX, 84580Y (10 October 2012); doi: 10.1117/12.928919
Show Author Affiliations
Anna-Karin Gustavsson, Univ. of Gothenburg (Sweden)
Caroline B. Adiels, Univ. of Gothenburg (Sweden)
Caroline B. Adiels, Univ. of Gothenburg (Sweden)
Mattias Goksör, Univ. of Gothenburg (Sweden)
Published in SPIE Proceedings Vol. 8458:
Optical Trapping and Optical Micromanipulation IX
Kishan Dholakia; Gabriel C. Spalding, Editor(s)
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