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Proceedings Paper

Patterned illumination for analysing neuronal function in 3D
Author(s): Mary Ann Go; Christian Stricker; Steve Redman; Hans Bachor; Vincent Ricardo Daria
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Paper Abstract

We use patterned 3D multi-spot illumination to perform neuronal multi-site stimulation in rat brain tissue. Using a spatial light modulator, we holograpically project 3D light fields for multi-site two-photon photolysis of caged neurotransmitters to generate synaptic inputs to a neuron. Controlled photostimulation of multiple synapses from various locations in the dendritic tree provides a way to analyze how neurons integrate multiple inputs. Our holographic projection setup is incorporated into a two-photon 3D imaging microscope for visualization and for accurate positioning of specific uncaging sites along the neuron's dendritic tree. We show two-photon images and the neuron's response to holographic photostimulation of synapses along dendrites.

Paper Details

Date Published: 8 May 2012
PDF: 6 pages
Proc. SPIE 8427, Biophotonics: Photonic Solutions for Better Health Care III, 842703 (8 May 2012); doi: 10.1117/12.923421
Show Author Affiliations
Mary Ann Go, The Australian National Univ. (Australia)
Christian Stricker, The Australian National Univ. (Australia)
Steve Redman, The Australian National Univ. (Australia)
Hans Bachor, The Australian National Univ. (Australia)
Vincent Ricardo Daria, The Australian National Univ. (Australia)

Published in SPIE Proceedings Vol. 8427:
Biophotonics: Photonic Solutions for Better Health Care III
Jürgen Popp; Wolfgang Drexler; Valery V. Tuchin; Dennis L. Matthews, Editor(s)

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