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Proceedings Paper

Towards a laser-integrated module for marker-free sorting of micrometer-sized particles in microfluidic channels
Author(s): Wolfgang Schwarz; Anna Bergmann; Antonio Jorge Márquez del Pino; Dietmar Wahl; Dieter Rimpf; Timo Mappes; Rainer Michalzik
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Paper Abstract

In recent years, microfluidic devices have become important tools for cell analysis in biology and medicine. They enable fast and inexpensive analysis with reduced consumption of analytes. However, for optical detection involving FACS (fluorescence-activated cell sorting), sample preparation by attaching an antibody-labeled fluorochrome to the cell is required. Cell tagging by fluorochromes is a mature technology but might affect cell viability and function. In this paper we present a novel concept for marker-free detection and first realization steps. We show the integration of a microfluidic chip and an electrically pumped GaAs-based oxide-confined VECSEL (vertical-extended- cavity surface-emitting laser). Particles in the microchannel flow through the laser resonator and induce a change of the cavity resonance, thus allowing sensitive detection to trigger a subsequent sorting process.

Paper Details

Date Published: 8 May 2012
PDF: 9 pages
Proc. SPIE 8427, Biophotonics: Photonic Solutions for Better Health Care III, 84270U (8 May 2012);
Show Author Affiliations
Wolfgang Schwarz, Ulm Univ. (Germany)
Anna Bergmann, Ulm Univ. (Germany)
Antonio Jorge Márquez del Pino, Ulm Univ. (Germany)
Dietmar Wahl, Ulm Univ. (Germany)
Dieter Rimpf, Karlsruhe Institute of Technology (Germany)
Timo Mappes, Karlsruhe Institute of Technology (Germany)
Rainer Michalzik, Ulm Univ. (Germany)

Published in SPIE Proceedings Vol. 8427:
Biophotonics: Photonic Solutions for Better Health Care III
Jürgen Popp; Wolfgang Drexler; Valery V. Tuchin; Dennis L. Matthews, Editor(s)

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