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Proceedings Paper

Label-free multimodal microspectroscopic differentiation of glioblastoma tumor model cell lines combined with multivariate data analysis
Author(s): Edwin Ostertag; Barbara Boldrini; Sabrina Luckow; Rudolf W. Kessler
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Paper Abstract

Glioblastoma multiforme represents a highly lethal brain tumor. A tumor model has been developed based on the U-251 MG cell line from a human explant. The tumor model simulates different malignancies by controlled expression of the tumor suppressor proteins PTEN and TP53 within the cell lines derived from the wild type. The cells from each different malignant cell line are grown on slides, followed by a paraformaldehyde fixation. UV / VIS and IR spectra are recorded in the cell nuclei. For the differentiation of the cell lines a principal component analysis (PCA) is performed. The PCA demonstrates a good separation of the tumor model cell lines both with UV / VIS spectroscopy and with IR spectroscopy.

Paper Details

Date Published: 8 May 2012
PDF: 10 pages
Proc. SPIE 8427, Biophotonics: Photonic Solutions for Better Health Care III, 84271U (8 May 2012); doi: 10.1117/12.922602
Show Author Affiliations
Edwin Ostertag, Reutlingen Research Institute (Germany)
Barbara Boldrini, Reutlingen Research Institute (Germany)
Sabrina Luckow, Reutlingen Research Institute (Germany)
Rudolf W. Kessler, Reutlingen Research Institute (Germany)

Published in SPIE Proceedings Vol. 8427:
Biophotonics: Photonic Solutions for Better Health Care III
Jürgen Popp; Wolfgang Drexler; Valery V. Tuchin; Dennis L. Matthews, Editor(s)

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