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Proceedings Paper

Infrared spectroscopic studies to understand the effect of drugs at molecular level
Author(s): Bhawana Singh; Rekha Gautam; Bhagawat Chandrasekar; Srabanti Rakshit; Vinay Kumar B. N.; Sivaraman Boopathy; Dipankar Nandi; Kumaravel Somasundaram; Siva Umapathy
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Paper Abstract

In the recent past, there have been enormous efforts to understand effect of drugs on human body. Prior to understand the effect of drugs on human body most of the experiments are carried out on cells or model organisms. Here we present our study on the effect of chemotherapeutic drugs on cancer cells and the acetaminophen (APAP) induced hepatotoxicity in mouse model. Histone deacetylase inhibitors (HDIs) have attracted attention as potential drug molecules for the treatment of cancer. These are the chemotherapeutic drugs which have indirect mechanistic action against cancer cells via acting against histone deacetylases (HDAC). It has been known that different HDAC enzymes are over-expressed in various types of cancers for example; HDAC1 is over expressed in prostate, gastric and breast carcinomas. Therefore, in order to optimise chemotherapy, it is important to determine the efficacy of various classes of HDAC inhibitor drugs against variety of over-expressed HDAC enzymes. In the present study, FTIR microspectroscopy has been employed to predict the acetylation and propionylation brought in by HDIs. The liver plays an important role in cellular metabolism and is highly susceptible to drug toxicity. APAP which is an analgesic and antipyretic drug is extensively used for therapeutic purposes and has become the most common cause of acute liver failure (ALF). In the current study, we have focused to understand APAP induced hepatotoxicity using FTIR microspectroscopy. In the IR spectrum the bands corresponding to glycogen, ester group and were found to be suitable markers to predict liver injury at early time point (0.5hr) due to APAP both in tissue and serum in comparison to standard biochemical assays. Our studies show the potential of FTIR spectroscopy as a rapid, sensitive and non invasive detection technique for future clinical diagnosis.

Paper Details

Date Published: 8 May 2012
PDF: 14 pages
Proc. SPIE 8427, Biophotonics: Photonic Solutions for Better Health Care III, 84271T (8 May 2012); doi: 10.1117/12.922402
Show Author Affiliations
Bhawana Singh, Indian Institute of Science (India)
Rekha Gautam, Indian Institute of Science (India)
Bhagawat Chandrasekar, Indian Institute of Science (India)
Srabanti Rakshit, Indian Institute of Science (India)
Vinay Kumar B. N., Indian Institute of Science (India)
Sivaraman Boopathy, Indian Institute of Science (India)
Dipankar Nandi, Indian Institute of Science (India)
Kumaravel Somasundaram, Indian Institute of Science (India)
Siva Umapathy, Indian Institute of Science (India)

Published in SPIE Proceedings Vol. 8427:
Biophotonics: Photonic Solutions for Better Health Care III
Jürgen Popp; Wolfgang Drexler; Valery V. Tuchin; Dennis L. Matthews, Editor(s)

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