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Proceedings Paper

Off-axis multispectral digital holographic microscope with partially coherent illumination
Author(s): Frank Dubois; Catherine Yourassowsky
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Paper Abstract

We developed a new off-axis digital holographic microscope (DHM), working in transmission with a RGB LED illumination. This partially coherent multi-wavenlenght source gives a low noise holograms and the intensity image quality is fully exploitable for microscopy purposes. We implemented a full off-axis configuration enables the recording of the holographic data in one shot. As we used for the first tests a monochromatic CCD camera, the holograms are recorded separately in each spectral channel. The holograms are individually processed by the Fourier method to obtain, in each color, the complex amplitudes and the corresponding intensities and optical phases. The color intensities are recombined to obtain a composite RGB color image. The digital holographic refocusing and the optical phase map computation are successfully demonstrated. The multi-wavelengths operation provides a significant improvement of the collected information for colored samples. The full one-shot color hologram recording should be available with a color camera.

Paper Details

Date Published: 4 May 2012
PDF: 6 pages
Proc. SPIE 8429, Optical Modelling and Design II, 84291E (4 May 2012); doi: 10.1117/12.922005
Show Author Affiliations
Frank Dubois, Univ. Libre de Bruxelles (Belgium)
Catherine Yourassowsky, Univ. Libre de Bruxelles (Belgium)

Published in SPIE Proceedings Vol. 8429:
Optical Modelling and Design II
Frank Wyrowski; John T. Sheridan; Jani Tervo; Youri Meuret, Editor(s)

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