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Proceedings Paper

Protein detection system based on 32x32 SPAD pixel array
Author(s): L. Pancheri; L. Pasquardini; E. Morganti; N. Massari; D. Stoppa; C. Collini; L. Lorenzelli; L. Lunelli; C. Pederzolli
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Paper Abstract

In this work, a compact low-cost system designed to detect low amounts of proteins in biological fluids is presented. The system, based on time-gated fluorescence detection principle, is composed by a Single-Photon Avalanche Diode (SPAD) pixel array, a LED excitation light source and a micro-machined reaction chamber coupled to a microfluidic network. A dual-site binding strategy based on DNA aptamers is used for target protein recognition. The microreactor, composed of an array of microwells covered with a transparent membrane, is functionalized with a primary aptamer, while a fluorescent-tagged secondary aptamer is used for the detection. Preliminary measurements demonstrate the feasibility of fluorescence lifetime detection to discriminate between different fluorophores. The detection of human thrombin protein in 300nM concentration is reported as a biological proof of principle of the biosensor.

Paper Details

Date Published: 9 May 2012
PDF: 8 pages
Proc. SPIE 8439, Optical Sensing and Detection II, 843913 (9 May 2012); doi: 10.1117/12.921782
Show Author Affiliations
L. Pancheri, Fondazione Bruno Kessler (Italy)
L. Pasquardini, Fondazione Bruno Kessler (Italy)
E. Morganti, Fondazione Bruno Kessler (Italy)
N. Massari, Fondazione Bruno Kessler (Italy)
D. Stoppa, Fondazione Bruno Kessler (Italy)
C. Collini, Fondazione Bruno Kessler (Italy)
L. Lorenzelli, Fondazione Bruno Kessler (Italy)
L. Lunelli, Fondazione Bruno Kessler (Italy)
C. Pederzolli, Fondazione Bruno Kessler (Italy)

Published in SPIE Proceedings Vol. 8439:
Optical Sensing and Detection II
Francis Berghmans; Anna Grazia Mignani; Piet De Moor, Editor(s)

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