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Proceedings Paper

Non-invasive label-free investigation and typing of head and neck cancers by multimodal nonlinear microscopy
Author(s): Tobias Meyer; Nadine Vogler; Benjamin Dietzek; Denis Akimov; Johanna Inhestern; Orlando Guntinas-Lichius; Jürgen Popp
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Paper Abstract

Early detection and typing of tumors is pressing matter in clinical research with important impacts for prognosis and successful treatment. Currently, staining is the golden standard in histopathology but requires surgical removal of tissue. In order to avoid resection of non-diseased tissue a non-invasive real-time imaging method is required which can be applied ideally intrasurgically. In this proceeding a combination of second harmonic generation (SHG), two photon excited fluorescence (TPEF) and coherent anti-Stokes Raman (CARS) imaging has been employed to investigate tissue sections of head and neck carcinomas focussing on laryngeal carcinoma. Primary laryngeal and other head and neck carcinomas consist to 99% of squamous cell carcinoma. By fusing the various imaging methods it is possible to measure the thickness of the epithelial cell layer as a marker for dysplastic or cancerous tissue degradation and to differentiate keratinizing and nonkeratininzing squamous cell carcinomas (SCC). As nonkeratinizing SCCs of the oropharynx correlate with a human papillomavirus (HPV) infection as a subentity of head and neck cancer, and HPV related tumors are associated with a better clinical prognosis, the differentiation between keratinizing and non-keratinizing forms of SCCs is of high diagnostic value. TPEF is capable of displaying cell nuclei, therefore, morphologic information as cell density, cell to cytoplasm ratio, size and shape of cell nuclei can be obtained. SHG - on the other hand - selectively reveals the collagen matrix of the connective tissue, which is useful for determination of tumor-islets boundaries within epithelial tissue - a prerequisite for precise resection. Finally CARS in the CH-stretching region visualizes the lipid content of the tissue, which can be correlated with the dysplastic grade of the tissue.

Paper Details

Date Published: 8 May 2012
PDF: 7 pages
Proc. SPIE 8427, Biophotonics: Photonic Solutions for Better Health Care III, 84270Z (8 May 2012); doi: 10.1117/12.921631
Show Author Affiliations
Tobias Meyer, Institut für Photonische Technologien e.V. (Germany)
Nadine Vogler, Institut für Photonische Technologien e.V. (Germany)
Benjamin Dietzek, Institut für Photonische Technologien e.V. (Germany)
Friedrich-Schiller-Univ. Jena (Germany)
Denis Akimov, Institut für Photonische Technologien e.V. (Germany)
Johanna Inhestern, Universitätsklinikum Jena (Germany)
Orlando Guntinas-Lichius, Universitätsklinikum Jena (Germany)
Jürgen Popp, Institut für Photonische Technologien e.V. (Germany)

Published in SPIE Proceedings Vol. 8427:
Biophotonics: Photonic Solutions for Better Health Care III
Jürgen Popp; Wolfgang Drexler; Valery V. Tuchin; Dennis L. Matthews, Editor(s)

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