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Proceedings Paper

Force and Raman spectroscopy of single red blood cell
Author(s): Saurabh Raj; Michal Wojdyla; Monica Marro Sanchez; Dmitri Petrov
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Paper Abstract

Viscoelastic and spectroscopic properties of single RBC are probed using dual beam optical tweezers and Raman techniques, respectively. Complex response function of cell was measured by means of one and two particles passive microrheology at different stretching states yielding local and overall mechanical properties of exactly the same human erythrocyte. The frequency dependent response function (measured up to 10 kHz) was corrected for the presence of the traps and spectral distribution of complex stiffness over controlled range of cell deformation is calculated and discussed. The presence of non-thermal sources of membrane motions is also explored based on comparison of passive and active microrheology experiments. In order to get insight into structural changes of RBC due to deformation, Raman spectra of single cell were recorded. Evolution of Raman bands with cell deformation was analyzed using sensitive 2D correlation method. The combination of force and Raman spectroscopy is promising and potentially very powerful method to establish essential linkages between structure, mechanical properties and functions of living cells.

Paper Details

Date Published: 8 May 2012
PDF: 15 pages
Proc. SPIE 8427, Biophotonics: Photonic Solutions for Better Health Care III, 842712 (8 May 2012); doi: 10.1117/12.921355
Show Author Affiliations
Saurabh Raj, ICFO - Institut de Ciències Fotòniques (Spain)
Michal Wojdyla, ICFO - Institut de Ciències Fotòniques (Spain)
Monica Marro Sanchez, ICFO - Institut de Ciències Fotòniques (Spain)
Dmitri Petrov, ICFO - Institut de Ciències Fotòniques (Spain)
Institució Catalana de Recerca i Estudis Avançats (Spain)

Published in SPIE Proceedings Vol. 8427:
Biophotonics: Photonic Solutions for Better Health Care III
Jürgen Popp; Wolfgang Drexler; Valery V. Tuchin; Dennis L. Matthews, Editor(s)

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