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Proceedings Paper

Rapid analysis of foodborne pathogens by surface-enhanced Raman spectroscopy
Author(s): Atanu Sengupta; Chetan Shende; Hermes Huang; Stuart Farquharson; Frank Inscore
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Paper Abstract

Foodborne diseases resulting from Campylobacter, Escherichia, Listeria, Salmonella, Shigella and Vibrio species affect as many as 76 million persons in the United States each year, resulting in 325,000 hospitalizations and 5,000 deaths. The challenge to preventing distribution and consumption of contaminated foods lies in the fact that just a few bacterial cells can rapidly multiply to millions, reaching infectious doses within a few days. Unfortunately, current methods used to detect these few cells rely on lengthy growth enrichment steps that take a similar amount of time (1 to 4 days). Consequently, there is a critical need for an analyzer that can rapidly extract and detect foodborne pathogens in 1-2 hours (not days), at 100 colony forming units per gram of food, and with a specificity that differentiates from indigenous microflora, so that false alarms are eliminated. In an effort to meet this need, we have been developing a sample system that extracts such pathogens from food, selectively binds these pathogens, and produces surface-enhanced Raman spectra (SERS). Here we present preliminary SERS measurements of Listeria and Salmonella.

Paper Details

Date Published: 4 May 2012
PDF: 13 pages
Proc. SPIE 8369, Sensing for Agriculture and Food Quality and Safety IV, 83690K (4 May 2012); doi: 10.1117/12.920856
Show Author Affiliations
Atanu Sengupta, Real-Time Analyzers, Inc. (United States)
Chetan Shende, Real-Time Analyzers, Inc. (United States)
Hermes Huang, Real-Time Analyzers, Inc. (United States)
Stuart Farquharson, Real-Time Analyzers, Inc. (United States)
Frank Inscore, Real-Time Analyzers, Inc. (United States)

Published in SPIE Proceedings Vol. 8369:
Sensing for Agriculture and Food Quality and Safety IV
Moon S. Kim; Shu-I Tu; Kuanglin Chao, Editor(s)

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