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Proceedings Paper

Development and testing of a fluorescence biosensor for glucose sensing
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Paper Abstract

Rapid, accurate, and minimally-invasive biosensors for glucose measurement have the potential to enhance management of diabetes mellitus and improve patient outcome in intensive care settings. Recent studies have indicated that implantable biosensors based on Förster Resonance Energy Transfer (FRET) can provide high sensitivity in quantifying glucose concentrations. However, standard approaches for determining the potential for interference from other biological constituents have not been established. The aim of this work was to design and optimize a FRET-based glucose sensor and assess its specificity to glucose. A sensor based on competitive binding between concanavalin A and dextran, labeled with long-wavelength acceptor and donor fluorophores, was developed. This process included optimization of dextran molecular weight and donor concentration, acceptor to donor ratio, and hydrogel concentration, as well as the number of polymer layers for encapsulation. The biosensor performance was characterized in terms of its response to clinically relevant glucose concentrations. The potential for interference and the development of test methods to evaluate this effect were studied using a potential clinical interferent, maltose. Results indicated that our biosensor had a prediction accuracy of better than 11% and that the robustness to maltose was highly dependent on glucose level.

Paper Details

Date Published: 16 May 2012
PDF: 11 pages
Proc. SPIE 8367, Smart Biomedical and Physiological Sensor Technology IX, 83670H (16 May 2012); doi: 10.1117/12.920699
Show Author Affiliations
Mamdouh Aloraefy, The Catholic Univ. of America (United States)
U.S. Food and Drug Administration (United States)
Joshua Pfefer, U.S. Food and Drug Administration (United States)
Jessica Ramella-Roman, The Catholic Univ. of America (United States)
Kim Sapsford, U.S. Food and Drug Administration (United States)

Published in SPIE Proceedings Vol. 8367:
Smart Biomedical and Physiological Sensor Technology IX
Brian M. Cullum; Eric S. McLamore, Editor(s)

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