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Proceedings Paper

Fabrication and characterization of a solid state nanopore with self-aligned carbon nanoelectrodes for molecular detection
Author(s): Patrick Spinney; Scott D. Collins; David G. Howitt; Rosemary L. Smith
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Paper Abstract

Rapid and cost-effective DNA sequencing is a pivotal prerequisite for the genomics era. Many of the recent advances in forensics, medicine, agriculture, taxonomy, and drug discovery have paralleled critical advances in DNA sequencing technology. Nanopore modalities for DNA sequencing have recently surfaced including the electrical interrogation of protein ion channels and/or solid-state nanopores during translocation of DNA. However to date, most of this work has met with mixed success. In this work, we present a unique nanofabrication strategy that realizes an artificial nanopore articulated with carbon electrodes to sense the current modulations during the transport of DNA through the nanopore. This embodiment overcomes most of the technical difficulties inherent in other artificial nanopore embodiments and present a versatile platform for the testing of DNA single nucleotide detection. Characterization of the device using gold nanoparticles, silica nanoparticles, lambda dsDNA and 16-mer ssDNA are presented. Although single molecule DNA sequencing is still not demonstrated, the device shows a path towards this goal.

Paper Details

Date Published: 3 May 2012
PDF: 10 pages
Proc. SPIE 8373, Micro- and Nanotechnology Sensors, Systems, and Applications IV, 83730P (3 May 2012); doi: 10.1117/12.919962
Show Author Affiliations
Patrick Spinney, Univ. of Maine (United States)
Scott D. Collins, Univ. of Maine (United States)
David G. Howitt, Univ. of California, Davis (United States)
Rosemary L. Smith, Univ. of Maine (United States)

Published in SPIE Proceedings Vol. 8373:
Micro- and Nanotechnology Sensors, Systems, and Applications IV
Thomas George; M. Saif Islam; Achyut Dutta, Editor(s)

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