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Proceedings Paper

3D imaging of tomato seeds using frequency domain optical coherence tomography
Author(s): Chuanmao Fan; Gang Yao
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Paper Abstract

A fast imaging system that can reveal internal sample structures is important for research and quality controls of seeds. Optical coherence tomography (OCT) is a non-invasive optical imaging technique that can acquire high speed, high resolution depth-resolved images in scattering samples. It has found numerous applications in studying various biological tissues and other materials in vivo. A few studies have reported the use of OCT in studying seed morphology. However, 3D imaging of internal seed structure has not been reported before. In this study, we used a frequency domain OCT system to image tomato seeds. The system has a central wavelength of 844nm with a 46.8 nm FWHM bandwidth. The requirement for depth scan was eliminated by using a Fourier domain implementation. The B-scan imaging speed was limited by the spectroscopic imaging CCD at 52 kHz. The calibrated system has a 6.7μm depth resolution and a 15.4μm lateral resolution. Our results show that major seed structures can be clearly visualized in OCT images.

Paper Details

Date Published: 4 May 2012
PDF: 6 pages
Proc. SPIE 8369, Sensing for Agriculture and Food Quality and Safety IV, 83690F (4 May 2012); doi: 10.1117/12.919347
Show Author Affiliations
Chuanmao Fan, Univ. of Missouri-Columbia (United States)
Gang Yao, Univ. of Missouri-Columbia (United States)

Published in SPIE Proceedings Vol. 8369:
Sensing for Agriculture and Food Quality and Safety IV
Moon S. Kim; Shu-I Tu; Kuanglin Chao, Editor(s)

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