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Proceedings Paper

Picosecond-resolved FRET on non-amplified DNA for identifying individuals genetically susceptible to type-1 diabetes
Author(s): Luca Nardo; Giovanna Tosi; Maria Bondani; Roberto Accolla; Alessandra Andreoni
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Paper Abstract

By tens-of-picosecond resolved fluorescence detection we study Förster resonance energy transfer between a donor and a black-hole-quencher bound at the 5'- and 3'-positions of an oligonucleotide probe matching the highly polymorphic region between codons 51 and 58 of the human leukocyte antigen DQB1 0201 allele, conferring susceptibility to type-1 diabetes. The probe is annealed with non-amplified genomic DNAs carrying either the 0201 sequence or other DQB1 allelic variants. We detect the longest-lived donor fluorescence in the case of hybridization with the 0201 allele and definitely faster and distinct decays for the other allelic variants, some of which are single-nucleotide polymorphic.

Paper Details

Date Published: 22 May 2012
PDF: 7 pages
Proc. SPIE 8375, Advanced Photon Counting Techniques VI, 837512 (22 May 2012); doi: 10.1117/12.919182
Show Author Affiliations
Luca Nardo, Univ. of Insubria (Italy)
CNISM (Italy)
Giovanna Tosi, Univ. of Insubria (Italy)
Maria Bondani, Institute for Photonics and Nanotechnologies (Italy)
CNISM (Italy)
Roberto Accolla, Univ. of Insubria (Italy)
Alessandra Andreoni, Univ. of Insubria (Italy)

Published in SPIE Proceedings Vol. 8375:
Advanced Photon Counting Techniques VI
Mark A. Itzler, Editor(s)

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