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Proceedings Paper

Highly scalable methods for exploiting a label with unknown location in order to orient a set of single-particle cryo electron microscopy images
Author(s): Cory J. Prust; Qiu Wang; Peter C. Doerschuk; John E. Johnson
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Paper Abstract

A highly scalable method for determining the projection orientation of each image in a set of cryo electron microscopy images of a labeled particle is proposed. The method relies on the presence of a label that is a sufficiently strong scatterer such that its 2-D location in each image can be restricted to at most a small number of sites by processing applied to each image individually. It is not necessary to know the 3-D location of the label on the particle. After first determining the possible locations of the label in the 2-D images in parallel, the information from all images is fused to determine the 3-D location of the label on the particle and then the 3-D location is used to determine the projection orientation for each image by processing each image individually. With projection orientations, many algorithms exist for computing the 3-D reconstruction. The performance of the algorithm is studied as a function of the label SNR.

Paper Details

Date Published: 10 February 2012
PDF: 6 pages
Proc. SPIE 8296, Computational Imaging X, 82960B (10 February 2012); doi: 10.1117/12.917838
Show Author Affiliations
Cory J. Prust, Milwaukee School of Engineering (United States)
Qiu Wang, Cornell Univ. (United States)
Peter C. Doerschuk, Cornell Univ. (United States)
John E. Johnson, The Scripps Research Institute (United States)

Published in SPIE Proceedings Vol. 8296:
Computational Imaging X
Charles A. Bouman; Ilya Pollak; Patrick J. Wolfe, Editor(s)

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