Elastic light single-scattering spectroscopy (ELSSS) system with a single optical fiber probe was employed to differentiate cancerous prostate tissue from non-cancerous prostate tissue ex-vivo just after radical prostatectomy. First, ELSSS spectra were acquired from cancerous prostate tissue to define its spectral features. Then, spectra were acquired from surgical margins of excised prostate tissue to detect positive surgical margins based on the spectral features of the spectra taken from cancerous prostate tissues. Of the total 128 tissue samples were evaluated from 18 patients by ELSSS system. Comparing of histopathology results and ELSSS measurements revealed that sign of the spectral slopes of cancerous prostate tissue is negative and non-cancerous tissue is positive in the wavelength range from 450 to 750 nm. Sign of the spectral slopes were used as a discrimination parameter between cancerous and non-cancerous prostate tissues. Based on the correlation between histopathology results and sign of the spectral slopes, ELSSS system differentiates cancerous prostate tissue from non-cancerous with a sensitivity of 0.97 and a specificity of 0.87.

Date Published: 11 October 2011
PDF: 6 pages
Proc. SPIE 8306, Photonics, Devices, and Systems V, 83060J (11 October 2011); doi: 10.1117/12.915799

Published in SPIE Proceedings Vol. 8306:
Photonics, Devices, and Systems V
Pavel Tománek; Dagmar Senderáková; Petr Páta, Editor(s)