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Proceedings Paper

Photonic crystal fiber monitors for intracellular ice formation
Author(s): Emily Battinelli; Mark Reimlinger; Rosalind Wynne
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Paper Abstract

An all-silica steering wheel photonic crystal fiber (SW-PCF) device with real-time analysis for cellular temperature sensing is presented. Results are provided for water-filled SW-PCF fibers experiencing cooling down near -40°C. Cellular temperature sensors with fast response times are of interest particularly to the study of cryopreservation, which has been influential in applications such as tissue preservation, food quality control, genetic engineering, as well as drug discovery and in- vitro toxin testing. Results of this investigation are relevant to detection of intracellular ice formation (IIF) and better understanding cell freezing at very low temperatures. IIF detection is determined as a function of absorption occurring within the core of the SW-PCF. The SW-PCF has a 3.3μm core diameter, 125μm outer diameter and steering wheel-like air hole pattern with triangular symmetry, with a 20μm radius. One end of a 0.6m length of the SW-PCF is placed between two thermoelectric coolers, filled with ~0.1μL water. This end is butt coupled to a 0.5m length of single mode fiber (SMF), the distal end of the fiber is then inserted into an optical spectrum analyzer. A near-IR light source is guided through the fiber, such that the absorption of the material in the core can be measured. Spectral characteristics demonstrated by the optical absorption of the water sample were present near the 1300-1700nm window region with strongest peaks at 1350, 1410 and 1460nm, further shifting of the absorption peaks is possible at cryogenic temperatures making this device suitable for IIF monitoring applications.

Paper Details

Date Published: 30 March 2012
PDF: 6 pages
Proc. SPIE 8346, Smart Sensor Phenomena, Technology, Networks, and Systems Integration 2012, 83460Q (30 March 2012); doi: 10.1117/12.914974
Show Author Affiliations
Emily Battinelli, Villanova Univ. (United States)
Mark Reimlinger, Villanova Univ. (United States)
Rosalind Wynne, Villanova Univ. (United States)

Published in SPIE Proceedings Vol. 8346:
Smart Sensor Phenomena, Technology, Networks, and Systems Integration 2012
Theodore E. Matikas, Editor(s)

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