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Proceedings Paper

Label-free multiphoton fluorescence imaging monitors metabolism in living primary human cells used for tissue engineering
Author(s): Leng-Chun Chen; William R. Lloyd; Shiuhyang Kuo; Cynthia L. Marcelo; Stephen E. Feinberg; Mary-Ann Mycek
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Paper Abstract

Fluorescence redox imaging was employed to monitor the metabolic activity of primary human oral keratinocytes prior to the development of tissue-engineered constructs. Keratinocytes with controlled culture conditions were treated with varying levels of chemical stimuli, resulting in differing cellular morphology, growth rate, and metabolic activity. Fluorescence images of keratinocytes were noninvasively acquired from endogenous intracellular metabolic fluorophores NAD(P)H and FAD. A redox ratio quantitatively analyzed each pair of images, showing that fluorescence redox imaging may be a novel technique to characterize live cell viability

Paper Details

Date Published: 9 February 2012
PDF: 6 pages
Proc. SPIE 8225, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues X, 82250H (9 February 2012); doi: 10.1117/12.910792
Show Author Affiliations
Leng-Chun Chen, Univ. of Michigan (United States)
William R. Lloyd, Univ. of Michigan (United States)
Shiuhyang Kuo, Univ. of Michigan (United States)
Cynthia L. Marcelo, Univ. of Michigan (United States)
Stephen E. Feinberg, Univ. of Michigan (United States)
Mary-Ann Mycek, Univ. of Michigan (United States)

Published in SPIE Proceedings Vol. 8225:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues X
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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