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Proceedings Paper

Fluorescence lifetime imaging microscopy (FLIM) studies of living primary human cells for applications in tissue regeneration
Author(s): William R. Lloyd; Leng-Chun Chen; Shiuhyang Kuo; Cynthia L. Marcelo; Stephen E. Feinberg; Mary-Ann Mycek
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Paper Abstract

Fluorescence lifetime imaging microscopy (FLIM) was employed to noninvasively characterize metabolic function in primary human oral keratinocytes used to develop functional engineered tissues. Living cells were compared under control culture conditions and systematic variations to investigate cellular function and viability. Nonlinear optical microscopy via two-photon excitation was employed to image cellular metabolic biomarkers NAD(P)H and FAD with thin optical sectioning and minimal out-of-focus fluorophore photobleaching. Novel post-processing FLIM algorithms were developed and tested. Results suggest that FLIM may provide useful information about live cell function and viability.

Paper Details

Date Published: 9 February 2012
PDF: 6 pages
Proc. SPIE 8226, Multiphoton Microscopy in the Biomedical Sciences XII, 82260E (9 February 2012); doi: 10.1117/12.910790
Show Author Affiliations
William R. Lloyd, Univ. of Michigan (United States)
Leng-Chun Chen, Univ. of Michigan (United States)
Shiuhyang Kuo, Univ. of Michigan (United States)
Cynthia L. Marcelo, Univ. of Michigan (United States)
Stephen E. Feinberg, Univ. of Michigan (United States)
Mary-Ann Mycek, Univ. of Michigan (United States)

Published in SPIE Proceedings Vol. 8226:
Multiphoton Microscopy in the Biomedical Sciences XII
Karsten König, Editor(s)

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