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Proceedings Paper

Optical mapping by low-cost instrumentation and disposable chemically induced nanochannels
Author(s): Philip J. R. Roche; Maurice C-K. Cheung; Lenore Beitel; Mark A. Trifiro M.D.; Vamsy P. Chodavarapu; Andrew G. Kirk
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Paper Abstract

The cost of fabrication and instrumentation presents a significant barrier to uptake of optical mapping as a tool for genomic investigation. Here a low cost optical instrumentation system to perform optical genomic mapping of DNA fragment restriction digestion by nanochannel confinement is presented. Specifically, the system is used for the detection of YOYO-1 labeled DNA within chemically formed nanochannels on a polystyrene chip. The formation of nanochannels on the polystyrene chip is achieved by solvent swelling of an injection moulded polystyrene substrate. The inverted microscope based system is compact and of low-cost but offers the sensitivity to detect individual fragments ranging from 0.56Kb to 9.4Kb of the λ-phage genome within a channels. Conformation of DNA within nanochannels driven by capillary flow is most consistent with modeled polymer extension in nanoslit.

Paper Details

Date Published: 2 February 2012
PDF: 9 pages
Proc. SPIE 8231, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications VIII, 82310K (2 February 2012); doi: 10.1117/12.910138
Show Author Affiliations
Philip J. R. Roche, McGill Univ. (Canada)
Maurice C-K. Cheung, McGill Univ. (Canada)
Lenore Beitel, Lady Davis Institute, Jewish General Hospital (Canada)
Mark A. Trifiro M.D., Lady Davis Institute, Jewish General Hospital (Canada)
Vamsy P. Chodavarapu, McGill Univ. (Canada)
Andrew G. Kirk, McGill Univ. (Canada)

Published in SPIE Proceedings Vol. 8231:
Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications VIII
Alexander N. Cartwright; Dan V. Nicolau, Editor(s)

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