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Proceedings Paper

Neuronal cell growth on polymeric scaffolds studied by CARS microscopy
Author(s): Annika Enejder; Helen Fink; Hans-Georg Kuhn
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Paper Abstract

For studies of neuronal cell integration and neurite outgrowth in polymeric scaffold materials as a future alternative for the treatment of damages in the neuronal system, we have developed a protocol employing CARS microscopy for imaging of neuronal networks. The benefits of CARS microscopy come here to their best use; (i) the overall three-dimensional (3D) arrangement of multiple cells and their neurites can be visualized without the need for chemical preparations or physical sectioning, potentially affecting the architecture of the soft, fragile scaffolds and (ii) details on the interaction between single cells and scaffold fibrils can be investigated by close-up images at sub-micron resolution. The establishment of biologically more relevant 3D neuronal networks in a soft hydrogel composed of native Extra Cellular Matrix (ECM) components was compared with conventional two-dimensional networks grown on a stiff substrate. Images of cells in the hydrogel scaffold reveal significantly different networking characteristics compared to the 2D networks, raising the question whether the functionality of neurons grown as layers in conventional cultivation dishes represents that of neurons in the central and peripheral nervous systems.

Paper Details

Date Published: 15 February 2012
PDF: 9 pages
Proc. SPIE 8226, Multiphoton Microscopy in the Biomedical Sciences XII, 82261W (15 February 2012); doi: 10.1117/12.910134
Show Author Affiliations
Annika Enejder, Chalmers Univ. of Technology (Sweden)
Helen Fink, Chalmers Univ. of Technology (Sweden)
Hans-Georg Kuhn, Univ. of Gothenburg (Sweden)


Published in SPIE Proceedings Vol. 8226:
Multiphoton Microscopy in the Biomedical Sciences XII
Karsten König, Editor(s)

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