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Proceedings Paper

Closed loop adaptive optics with a laser guide star for biological light microscopy
Author(s): Christopher D. Saunter; Cyril Bourgenot; John M. Girkin; Gordon D. Love
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Paper Abstract

We report on the development of a widefield microscope that achieves adaptive optics correction through the use of a wavefront sensor observing an artificial laser guide star induced within the sample. By generating this guide star at arbitrary positions and depths within the sample we allow the delivery of high-resolution images. This approach delivers much faster AO correction than image optimization techniques, and allows the use of AO with fluorescent imaging modalities without generating excessive photo-toxic damage in the sample, or inducing significant photo-bleaching in the flurophore molecules.

Paper Details

Date Published: 15 February 2012
PDF: 6 pages
Proc. SPIE 8253, MEMS Adaptive Optics VI, 82530J (15 February 2012); doi: 10.1117/12.909927
Show Author Affiliations
Christopher D. Saunter, Durham Univ. (United Kingdom)
Cyril Bourgenot, Durham Univ. (United Kingdom)
John M. Girkin, Durham Univ. (United Kingdom)
Gordon D. Love, Durham Univ. (United Kingdom)

Published in SPIE Proceedings Vol. 8253:
MEMS Adaptive Optics VI
Scot S. Olivier; Thomas G. Bifano; Joel Kubby, Editor(s)

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