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Proceedings Paper

Improved fabrication of zero-mode waveguides for monitoring specific molecular dynamics in living cells
Author(s): Chia-Fen Hsieh; Shui-Chin Lai; Chih-Ting Chen; Yii-Lih Lin; Kuo-Tang Liao; Lesser-Rojas Leonardo; K. K. Sriram; Shengqin Wang; Po-Chieh Chiang; Chia-Fu Chou
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Paper Abstract

Zero-mode waveguides (ZMWs) are optical nanostructures to confine fluorescent excitation within sub-diffraction volumes and are commonly used for single-molecule analysis. However, the conventional ZMWs with aluminum film on fused silica have limitations on living cell studies. The same surfaces composed of hydroxyl group inside and outside each ZMW restrict specific surface functionalization. The sharp-cylinder shaped and rough edge of each ZMW produces a steric interference of molecular dynamics on cell membrane. In this study, selectively surface functionalization inside and outside of each ZMW was achieved with tri-metal-layer film on fused silica. Moreover, bowl-shaped and smooth edge of each ZMW was manufactured in large area. The improvement of ZMWs provides a broad way for monitoring molecular dynamics in living cells.

Paper Details

Date Published: 2 February 2012
PDF: 7 pages
Proc. SPIE 8231, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications VIII, 82310W (2 February 2012); doi: 10.1117/12.909797
Show Author Affiliations
Chia-Fen Hsieh, Academia Sinica (Taiwan)
Shui-Chin Lai, Academia Sinica (Taiwan)
Chih-Ting Chen, Academia Sinica (Taiwan)
Yii-Lih Lin, Academia Sinica (Taiwan)
National Taiwan Univ. (Taiwan)
Kuo-Tang Liao, Academia Sinica (Taiwan)
Lesser-Rojas Leonardo, Academia Sinica (Taiwan)
National Tsing Hua Univ. (Taiwan)
K. K. Sriram, Academia Sinica (Taiwan)
National Tsing Hua Univ. (Taiwan)
Shengqin Wang, Academia Sinica (Taiwan)
Po-Chieh Chiang, Academia Sinica (Taiwan)
Chia-Fu Chou, Academia Sinica (Taiwan)

Published in SPIE Proceedings Vol. 8231:
Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications VIII
Alexander N. Cartwright; Dan V. Nicolau, Editor(s)

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