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Proceedings Paper

Multidepth imaging by chromatic dispersion confocal microscopy
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Paper Abstract

Confocal microscopy has shown potential as an imaging technique to detect precancer. Imaging cellular features throughout the depth of epithelial tissue may provide useful information for diagnosis. However, the current in vivo axial scanning techniques for confocal microscopy are cumbersome, time-consuming, and restrictive when attempting to reconstruct volumetric images acquired in breathing patients. Chromatic dispersion confocal microscopy (CDCM) exploits severe longitudinal chromatic aberration in the system to axially disperse light from a broadband source and, ultimately, spectrally encode high resolution images along the depth of the object. Hyperchromat lenses are designed to have severe and linear longitudinal chromatic aberration, but have not yet been used in confocal microscopy. We use a hyperchromat lens in a stage scanning confocal microscope to demonstrate the capability to simultaneously capture information at multiple depths without mechanical scanning. A photonic crystal fiber pumped with a 830nm wavelength Ti:Sapphire laser was used as a supercontinuum source, and a spectrometer was used as the detector. The chromatic aberration and magnification in the system give a focal shift of 140μm after the objective lens and an axial resolution of 5.2-7.6μm over the wavelength range from 585nm to 830nm. A 400x400x140μm3 volume of pig cheek epithelium was imaged in a single X-Y scan. Nuclei can be seen at several depths within the epithelium. The capability of this technique to achieve simultaneous high resolution confocal imaging at multiple depths may reduce imaging time and motion artifacts and enable volumetric reconstruction of in vivo confocal images of the epithelium.

Paper Details

Date Published: 28 February 2012
PDF: 6 pages
Proc. SPIE 8214, Advanced Biomedical and Clinical Diagnostic Systems X, 82140L (28 February 2012); doi: 10.1117/12.909175
Show Author Affiliations
Cory A. Olsovsky, Texas A&M Univ. (United States)
Ryan L. Shelton, Texas A&M Univ. (United States)
Meagan A. Saldua, Texas A&M Univ. (United States)
Oscar Carrasco-Zevallos, Texas A&M Univ. (United States)
Brian E. Applegate, Texas A&M Univ. (United States)
Kristen C. Maitland, Texas A&M Univ. (United States)

Published in SPIE Proceedings Vol. 8214:
Advanced Biomedical and Clinical Diagnostic Systems X
Tuan Vo-Dinh; Anita Mahadevan-Jansen; Warren S. Grundfest M.D., Editor(s)

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