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Proceedings Paper

Imaging dynamic processes using fiber laser optical-resolution photoacoustic microscopy
Author(s): Wei Shi; Peng Shao; Parsin Hajireza; Alexander Forbrich; Roger J. Zemp
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Paper Abstract

Recently we have reported in vivo near-real-time volumetric optical-resolution photoacoustic microscopy (OR-PAM) using a high pulse-repetition-rate (PRR) nanosecond fiber-laser to realize 2 volumetric image frames per second (fps) within 1mm × 1mm field of view (FOV). Based on our previous OR-PAM system, we are developing a label-free realtime OR-PAM system in reflection mode for higher frame-rates. The system permits imaging of microcirculation hemodynamics, and helps make the technology easier to use for biologists, providing real-time feedback for focusing and positioning. Using a nanosecond-pulsed 532-nm fiber laser combined with fast-scanning mirrors, our proposed system demonstrated its capability of sustained in vivo imaging of horizontal and vertical translation at 0.5 fps for 1mm × 1mm FOV (400 × 400 pixels). Also, real-time in vivo imaging of blood flow at 30 fps for 250μm × 250μm FOV (100 × 100 pixels) was demonstrated. It is anticipated that the real-time nature of the system should prove important in clinical and preclinical adaption, and may prove useful for functional brain imaging studies.

Paper Details

Date Published: 23 February 2012
PDF: 6 pages
Proc. SPIE 8223, Photons Plus Ultrasound: Imaging and Sensing 2012, 82230Y (23 February 2012);
Show Author Affiliations
Wei Shi, Univ. of Alberta (Canada)
Peng Shao, Univ. of Alberta (Canada)
Parsin Hajireza, Univ. of Alberta (Canada)
Alexander Forbrich, Univ. of Alberta (Canada)
Roger J. Zemp, Univ. of Alberta (Canada)

Published in SPIE Proceedings Vol. 8223:
Photons Plus Ultrasound: Imaging and Sensing 2012
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)

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