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Proceedings Paper

Clinical multiphoton and CARS microscopy
Author(s): H. G. Breunig; M. Weinigel; M. E. Darvin; J. Lademann; K. König
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Paper Abstract

We report on clinical CARS imaging of human skin in vivo with the certified hybrid multiphoton tomograph CARSDermaInspect. The CARS-DermaInspect provides simultaneous imaging of non-fluorescent intradermal lipid and water as well as imaging of two-photon excited fluorescence from intrinsic molecules. Two different excitation schemes for CARS imaging have been realized: In the first setup, a combination of fs oscillator and optical parametric oscillator provided fs-CARS pump and Stokes pulses, respectively. In the second setup a fs oscillator was combined with a photonic crystal fiber which provided a broadband spectrum. A spectral range out of the broadband-spectrum was selected and used for CARS excitation in combination with the residual fs-oscillator output. In both setups, in addition to CARS, single-beam excitation was used for imaging of two-photon excited fluorescence and second harmonic generation signals. Both CARS-excitation systems were successfully used for imaging of lipids inside the skin in vivo.

Paper Details

Date Published: 9 February 2012
PDF: 7 pages
Proc. SPIE 8226, Multiphoton Microscopy in the Biomedical Sciences XII, 822623 (9 February 2012); doi: 10.1117/12.908187
Show Author Affiliations
H. G. Breunig, JenLab GmbH (Germany)
M. Weinigel, JenLab GmbH (Germany)
M. E. Darvin, Charité Univ. Berlin (Germany)
J. Lademann, Charité Univ. Berlin (Germany)
K. König, JenLab GmbH (Germany)
Saarland Univ. (Germany)

Published in SPIE Proceedings Vol. 8226:
Multiphoton Microscopy in the Biomedical Sciences XII
Karsten König, Editor(s)

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