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Proceedings Paper

Multi-functional stage-scanning fluorescence micro/nanoscope for single-lipid dynamics
Author(s): Li-Ling Yang; Chia-Fen Hsieh; Yi-Ren Chang; Jie-Pan Shen; Yu-Chung Chang; Chia-Fu Chou
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Paper Abstract

We combine pulsed laser, supercontinuum radiation source and fast single-photon counting peripherals to obtain a multifunctional micro/nano-scope. This provides us with better spatial and temporal resolution to observe fast dynamics. Performing fluorescence correlation spectroscopy for fast dynamics (<μs) with sub-diffraction limit resolution to observe the initial state of single-lipid dynamics in supported lipid bilayers and living cells is our goal. Lipid raft serves as a platform for recruiting signaling components of effective signal transduction. However, the dynamics of sub-200nm rapidly aggregated lipid rafts are still not elucidated in living cells. We here report our recent progress on the construction of this multi-functional stage-scanning fluorescence micro/nanoscope for single-lipid dynamics study.

Paper Details

Date Published: 13 February 2012
PDF: 7 pages
Proc. SPIE 8228, Single Molecule Spectroscopy and Superresolution Imaging V, 822804 (13 February 2012); doi: 10.1117/12.908179
Show Author Affiliations
Li-Ling Yang, Academia Sinica (Taiwan)
Chia-Fen Hsieh, Academia Sinica (Taiwan)
Yi-Ren Chang, Academia Sinica (Taiwan)
Jie-Pan Shen, Academia Sinica (Taiwan)
Yu-Chung Chang, National Changhua Univ. of Education (Taiwan)
Chia-Fu Chou, Academia Sinica (Taiwan)

Published in SPIE Proceedings Vol. 8228:
Single Molecule Spectroscopy and Superresolution Imaging V
Jörg Enderlein; Zygmunt Karol Gryczynski; Rainer Erdmann; Felix Koberling; Ingo Gregor, Editor(s)

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