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Proceedings Paper

Prospective gating for 3D imaging of the beating zebrafish heart in embryonic development studies
Author(s): J. M. Taylor; C. D Saunter; G. D. Love; J. M. Girkin
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Paper Abstract

We demonstrate the use of prospective gating from continuously acquired brightfield images of zebrafish embryos to trigger the acquisition of fluorescence images with the heart at a precisely selected position in its cycle. The laser exposure of the sample is reduced by an order of magnitude compared to alternative techniques which acquire many separate fluorescence images for each section before selecting the most appropriate ones to build up a consistent 3D image stack. We present results obtained using our SPIM system including 3D reconstructions of the living, beating heart, acquired using optical gating without the need for any pharmacological or electrophysiological intervention, and discuss possible wider applications of our technique.

Paper Details

Date Published: 2 February 2012
PDF: 7 pages
Proc. SPIE 8227, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIX, 822716 (2 February 2012); doi: 10.1117/12.908164
Show Author Affiliations
J. M. Taylor, Durham Univ. (United Kingdom)
C. D Saunter, Durham Univ. (United Kingdom)
G. D. Love, Durham Univ. (United Kingdom)
J. M. Girkin, Durham Univ. (United Kingdom)

Published in SPIE Proceedings Vol. 8227:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIX
Jose-Angel Conchello; Carol J. Cogswell; Tony Wilson; Thomas G. Brown, Editor(s)

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