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Proceedings Paper

Surface plasmon enhanced-field fluorescence biosensor for point-of-care testing using fluorescent nanoparticles
Author(s): Kazuyoshi Horii; Toshihito Kimura; Hisashi Ohtsuka; Noriyuki Kasagi; Tomoya Oohara; Tadahiro Matsuno; Masashi Hakamata; Akihiro Komatsu; Tomonari Sendai
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Paper Abstract

An optical biosensor system using surface-plasmon field-enhanced fluorescence has been developed, which allows high sensitivity and fast measurement available. Intensity of fluorophores in SPFS is highly dependent upon the distance from metal surface. The resonant evanescent electric field excites fluorophores within the penetration area. On the other hand, fluorescence quenching in close proximity to a metal surface interfere with the excitation. We have developed a new technology for fluorescent nanoparticles that could receive the energy from metal surface effectively. This enables technology of detecting strong and stable SPFS signals, as well as homogeneous assay method that allows us to eliminate binding/free separation process for unreacted fluorescent particles. A rate assay method has also been employed, which resolves affect from diffusion-limited access, in order to realize a fast surface immunoreaction in a microchannel. Taking advantage of these two developments, as eliminating an enzyme response process such as CLEIA, our system reaches much faster reaction time of 2 minutes to detect thyroid stimulating hormone (TSH) of canine serum sample at 0.1ng/mL. We believe our system with these new technologies is a powerful tool for in-vitro diagnosis which meets various clinical requirements.

Paper Details

Date Published: 6 February 2012
PDF: 8 pages
Proc. SPIE 8234, Plasmonics in Biology and Medicine IX, 82340V (6 February 2012); doi: 10.1117/12.908099
Show Author Affiliations
Kazuyoshi Horii, FUJIFILM Corp. (Japan)
Toshihito Kimura, FUJIFILM Corp. (Japan)
Hisashi Ohtsuka, FUJIFILM Corp. (Japan)
Noriyuki Kasagi, FUJIFILM Corp. (Japan)
Tomoya Oohara, FUJIFILM Corp. (Japan)
Tadahiro Matsuno, FUJIFILM Corp. (Japan)
Masashi Hakamata, FUJIFILM Corp. (Japan)
Akihiro Komatsu, FUJIFILM Corp. (Japan)
Tomonari Sendai, FUJIFILM Corp. (Japan)

Published in SPIE Proceedings Vol. 8234:
Plasmonics in Biology and Medicine IX
Tuan Vo-Dinh; Joseph R. Lakowicz, Editor(s)

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