
Proceedings Paper
Multimodal light-sheet microscopy for fluorescence live imagingFormat | Member Price | Non-Member Price |
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Paper Abstract
Light-sheet microscopy, it is known as single plane illumination microscope (SPIM), is a fluorescence imaging
technique which can avoid phototoxic effects to living cells and gives high contrast and high spatial resolution by optical
sectioning with light-sheet illumination in developmental biology. We have been developed a multifunctional light-sheet
fluorescence microscopy system with a near infrared femto-second fiber laser, a high sensitive image sensor and a high
throughput spectrometer. We performed that multiphoton fluorescence images of a transgenic fish and a mouse embryo
were observed on the light-sheet microscope. As the results, two photon images with high contrast and high spatial
resolution were successfully obtained in the microscopy system. The system has multimodality, not only mutiphoton
fluorescence imaging, but also hyperspectral imaging, which can be applicable to fluorescence unmixing analysis and
Raman imaging. It enables to obtain high specific and high throughput molecular imaging in vivo and in vitro.
Paper Details
Date Published: 2 February 2012
PDF: 6 pages
Proc. SPIE 8227, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIX, 82271H (2 February 2012); doi: 10.1117/12.907937
Published in SPIE Proceedings Vol. 8227:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIX
Jose-Angel Conchello; Carol J. Cogswell; Tony Wilson; Thomas G. Brown, Editor(s)
PDF: 6 pages
Proc. SPIE 8227, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIX, 82271H (2 February 2012); doi: 10.1117/12.907937
Show Author Affiliations
Y. Oshima, Ehime Univ. Hospital (Japan)
National Institute for Basic Biology (Japan)
H. Kajiura-Kobayashi, National Institute for Basic Biology (Japan)
National Institute for Basic Biology (Japan)
H. Kajiura-Kobayashi, National Institute for Basic Biology (Japan)
S. Nonaka, National Institute for Basic Biology (Japan)
Published in SPIE Proceedings Vol. 8227:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIX
Jose-Angel Conchello; Carol J. Cogswell; Tony Wilson; Thomas G. Brown, Editor(s)
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