
Proceedings Paper
Control of burn wound sepsis in rats by methylene blue-mediated photodynamic treatmentFormat | Member Price | Non-Member Price |
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Paper Abstract
Control of wound sepsis is an important challenge in traumatology. However, increase in the drug-resistant bacteria
makes this challenge considerably difficult in recent years. In this study, we attempted to control burn wound sepsis in
rats by photodynamic treatment, which has been reported to be effective against some drug-resistant bacteria. A 20%
TBSA (total body surface area) full-thickness burn was made in rat dorsal skin, and five days after injury, a suspension
of P. aeruginosa was applied to the wound surface. At 30 min after infection, a methylene blue (MB) solution was
applied to the wound surface; 5 min afterwards, the wound was illuminated with a 665-nm light emitting diode (LED)
array for 10 min. This treatment (application of MB and illumination) was repeated 3 times successively. The averaged
light intensity on the wound surface was 3.3 mW/cm2, the corresponding total light dose being 5.9 J/cm2. One week after
injury, the numbers of bacteria in the blood and liver were counted by colony forming assay. In the liver, the number of
bacteria of the treated group was significantly lower than that of the sham control group without photodynamic treatment.
In the blood, no bacteria were detected in the treated group, while a certain amount of bacteria was detected in the
control group. These results demonstrate the efficacy of MB-mediated PDT with a red LED array to control burn wound
sepsis.
Paper Details
Date Published: 9 March 2012
PDF: 6 pages
Proc. SPIE 8210, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXI, 82100V (9 March 2012); doi: 10.1117/12.907643
Published in SPIE Proceedings Vol. 8210:
Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXI
David H. Kessel; Tayyaba Hasan, Editor(s)
PDF: 6 pages
Proc. SPIE 8210, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXI, 82100V (9 March 2012); doi: 10.1117/12.907643
Show Author Affiliations
Hiroyuki Hasegawa, Keio Univ. (Japan)
Shunichi Sato, National Defense Medical College (Japan)
Satoko Kawauchi, National Defense Medical College (Japan)
Daizoh Saitoh, National Defense Medical College (Japan)
Shunichi Sato, National Defense Medical College (Japan)
Satoko Kawauchi, National Defense Medical College (Japan)
Daizoh Saitoh, National Defense Medical College (Japan)
Nariyoshi Shinomiya, National Defense Medical College (Japan)
Hiroshi Ashida, National Defense Medical College (Japan)
Mitsuhiro Terakawa, Keio Univ. (Japan)
Hiroshi Ashida, National Defense Medical College (Japan)
Mitsuhiro Terakawa, Keio Univ. (Japan)
Published in SPIE Proceedings Vol. 8210:
Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXI
David H. Kessel; Tayyaba Hasan, Editor(s)
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