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Proceedings Paper

Control of burn wound sepsis in rats by methylene blue-mediated photodynamic treatment
Author(s): Hiroyuki Hasegawa; Shunichi Sato; Satoko Kawauchi; Daizoh Saitoh; Nariyoshi Shinomiya; Hiroshi Ashida; Mitsuhiro Terakawa
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Paper Abstract

Control of wound sepsis is an important challenge in traumatology. However, increase in the drug-resistant bacteria makes this challenge considerably difficult in recent years. In this study, we attempted to control burn wound sepsis in rats by photodynamic treatment, which has been reported to be effective against some drug-resistant bacteria. A 20% TBSA (total body surface area) full-thickness burn was made in rat dorsal skin, and five days after injury, a suspension of P. aeruginosa was applied to the wound surface. At 30 min after infection, a methylene blue (MB) solution was applied to the wound surface; 5 min afterwards, the wound was illuminated with a 665-nm light emitting diode (LED) array for 10 min. This treatment (application of MB and illumination) was repeated 3 times successively. The averaged light intensity on the wound surface was 3.3 mW/cm2, the corresponding total light dose being 5.9 J/cm2. One week after injury, the numbers of bacteria in the blood and liver were counted by colony forming assay. In the liver, the number of bacteria of the treated group was significantly lower than that of the sham control group without photodynamic treatment. In the blood, no bacteria were detected in the treated group, while a certain amount of bacteria was detected in the control group. These results demonstrate the efficacy of MB-mediated PDT with a red LED array to control burn wound sepsis.

Paper Details

Date Published: 9 March 2012
PDF: 6 pages
Proc. SPIE 8210, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXI, 82100V (9 March 2012); doi: 10.1117/12.907643
Show Author Affiliations
Hiroyuki Hasegawa, Keio Univ. (Japan)
Shunichi Sato, National Defense Medical College (Japan)
Satoko Kawauchi, National Defense Medical College (Japan)
Daizoh Saitoh, National Defense Medical College (Japan)
Nariyoshi Shinomiya, National Defense Medical College (Japan)
Hiroshi Ashida, National Defense Medical College (Japan)
Mitsuhiro Terakawa, Keio Univ. (Japan)

Published in SPIE Proceedings Vol. 8210:
Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXI
David H. Kessel; Tayyaba Hasan, Editor(s)

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