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Proceedings Paper

Fluorescence intensity fluctuations in single-molecule polarization sensitive measurements
Author(s): Lior Turgeman; Dror Fixler
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Paper Abstract

A general framework to include fluctuations in the single molecule fluorescence intensity (FI) signal due to random changes in molecule dipole orientation was introduced at Optics Express (21, 2007). By assuming continuous changes in dipole orientation described by Brownian rotational diffusion, this research derives the probability density function (PDF) equation of FI fluctuations. Solution of the proposed equation for several limiting cases and different correlation times yields the short time behavior of FI fluctuations. Monte Carlo simulations results, in accordance with those found in theory will be presented during our talk.

Paper Details

Date Published: 13 February 2012
PDF: 13 pages
Proc. SPIE 8228, Single Molecule Spectroscopy and Superresolution Imaging V, 82280I (13 February 2012); doi: 10.1117/12.907270
Show Author Affiliations
Lior Turgeman, Bar-Ilan Univ. (Israel)
Dror Fixler, Bar-Ilan Univ. (Israel)

Published in SPIE Proceedings Vol. 8228:
Single Molecule Spectroscopy and Superresolution Imaging V
Jörg Enderlein; Zygmunt Karol Gryczynski; Rainer Erdmann; Felix Koberling; Ingo Gregor, Editor(s)

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