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Proceedings Paper

Coherent Raman scattering microscopy for label-free imaging of live amphioxus
Author(s): Zhilong Yu; Tao Chen; Xiannian Zhang; Jie Shen; Junyuan Chen; Yanyi Huang
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Paper Abstract

The existence of notochord distinguishes chordates from other phyla. Amphioxus is the only animal that keeps notochord during the whole life. Notochord is a unique organ for amphioxus, with its vertically arranged muscular notochordal plates, which is different from notochords in embryos of other chordates. We use stimulated Raman scattering (SRS) microscopy as a non-invasive technique to image the chemical components in amphioxus notochord. SRS provides chemical specificity as spontaneous Raman does and offers a higher sensitivity for fast acquisition. Unlike coherent anti- Stokes Raman scattering (CARS) microscopy, SRS microscopy doesn't have non-resonant background and can better differentiate different components in the specimen. We verify that the notochord is a protein-rich organ, which agrees well with the result of conventional staining methods. Detailed structures in notochordal plates and notochordal sheath are revealed by SRS microscopy with diffraction limited resolution. Our experiment shows that SRS microscopy is an excellent imaging tool for biochemical research with its intrinsic chemical selectivity, high spatiotemporal resolution and native 3D optical sectioning ability.

Paper Details

Date Published: 16 February 2012
PDF: 8 pages
Proc. SPIE 8226, Multiphoton Microscopy in the Biomedical Sciences XII, 82261V (16 February 2012); doi: 10.1117/12.907025
Show Author Affiliations
Zhilong Yu, Peking Univ. (China)
Tao Chen, Peking Univ. (China)
Xiannian Zhang, Peking Univ. (China)
Jie Shen, Peking Univ. (China)
Junyuan Chen, Nanjing Univ. (China)
Yanyi Huang, Peking Univ. (China)

Published in SPIE Proceedings Vol. 8226:
Multiphoton Microscopy in the Biomedical Sciences XII
Karsten König, Editor(s)

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