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Proceedings Paper

Two-photon excited fluorescence spectroscopy and imaging of melanin in vitro and in vivo
Author(s): Tatiana B. Krasieva; Feng Liu; Chung-Ho Sun; Yu Kong; Mihaela Balu; Frank L. Meyskens; Bruce J. Tromberg
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Paper Abstract

The ability to detect early melanoma non-invasively would improve clinical outcome and reduce mortality. Recent advances in two-photon excited fluorescence (TPEF) in vivo microscopy offer a powerful tool in early malignant melanoma diagnostics. The goal of this work was to develop a TPEF optical index for measuring relative concentrations of eumelanin and pheomelanin since ex vivo studies show that changes in this ratio have been associated with malignant transformation. We acquired TPEF emission spectra (λex=1000 nm) of melanin from several specimens, including human hair, malignant melanoma cell lines, and normal melanocytes and keratinocytes in different skin layers (epidermis, papillary dermis) in five healthy volunteers in vivo. We found that the pheomelanin emission peaks at around 620 nm and is blue-shifted from the eumelanin with broad maximum at 640-680nm. We defined "optical melanin index" (OMI) as a ratio of fluorescence signal intensities measured at 645 nm and 615nm. The measured OMI for a melanoma cell line MNT-1 was 1.6±0.2. The MNT-46 and MNT-62 lines (Mc1R gene knockdown) showed an anticipated change in melanins production ratio and had OMI of 0.55±0.05 and 0.17±0.02, respectively, which strongly correlated with HPLC data obtained for these lines. Average OMI measured for basal cells layers (melanocytes and keratinocytes) in normal human skin type I, II-III (not tanned and tanned) in vivo was 0.5, 1.05 and 1.16 respectively. We could not dependably detect the presence of pheomelanin in highly pigmented skin type V-VI. These data suggest that a non-invasive TPEF index could potentially be used for rapid melanin ratio characterization both in vitro and in vivo, including pigmented lesions.

Paper Details

Date Published: 9 February 2012
PDF: 7 pages
Proc. SPIE 8226, Multiphoton Microscopy in the Biomedical Sciences XII, 82262S (9 February 2012); doi: 10.1117/12.906490
Show Author Affiliations
Tatiana B. Krasieva, Beckman Laser Institute and Medical Clinic (United States)
Feng Liu, Univ. of California, Irvine (United States)
Chung-Ho Sun, Beckman Laser Institute and Medical Clinic (United States)
Yu Kong, Xi'an Jiaotong Univ. (China)
Mihaela Balu, Beckman Laser Institute and Medical Clinic (United States)
Frank L. Meyskens, Univ. of California, Irvine (United States)
Bruce J. Tromberg, Beckman Laser Institute and Medical Clinic (United States)

Published in SPIE Proceedings Vol. 8226:
Multiphoton Microscopy in the Biomedical Sciences XII
Karsten König, Editor(s)

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