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Proceedings Paper

Chemical and biomolecule patterning on 2D surfaces using atmospheric pressure microcavity plasma array devices
Author(s): Sameer A. Al-Bataineh; Endre J. Szili; Gillies Desmet; Paul Ruschitzka; Philipp J. Gruner; Craig Priest; Nicolas H. Voelcker; David A. Steele; Robert D. Short; Hans J. Griesser
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Paper Abstract

This paper presents a method for chemical and biomolecule patterning on planar (2D) surfaces using atmospheric pressure microplasmas. Spatially controlled surface modification is important for the development of emerging technologies such as microfluidic lab-on-a-chip devices, biosensors and other diagnostics tools. A non-fouling layer of poly(N-isopropylacrylamide) aldehyde (pNIPAM-ald) polymer, grafted onto heptylamine plasma polymer (HApp) modified silicon substrates, was used to achieve this goal. The non-fouling behaviour of the pNIPAM-ald coating was investigated at a temperature below its lower critical solution temperature (LCST) using human serum albumin (HSA). XPS and ToF-SIMS were used to characterise the plasma polymer coating and its subsequent modification with pNIPAM-ald before and after HSA adsorption. A 7 x 7 microcavity plasma array device (each cavity had a 250 Νm diameter and was separated by 500 μm) was used for microplasma patterning. In a non-contact mode, helium microplasma treatment of the pNIPAM-ald coating was carried out for 60 s. The polymer coating was removed from regions directly exposed to microplasma cavities, as shown by ToF-SIMS. Microplasma treated regions were able to support the adsorption of fluorescently-labelled streptavidin whereas the rest of the coating was still non-fouling. This approach therefore resulted in spatially separated areas of immobilised protein.

Paper Details

Date Published: 23 December 2011
PDF: 11 pages
Proc. SPIE 8204, Smart Nano-Micro Materials and Devices, 82043H (23 December 2011); doi: 10.1117/12.903304
Show Author Affiliations
Sameer A. Al-Bataineh, Univ. of South Australia (Australia)
Endre J. Szili, Univ. of South Australia (Australia)
Gillies Desmet, Univ. of South Australia (Australia)
Paul Ruschitzka, Univ. of South Australia (Australia)
Philipp J. Gruner, Univ. of South Australia (Australia)
Craig Priest, Univ. of South Australia (Australia)
Nicolas H. Voelcker, Flinders Univ. (Australia)
David A. Steele, Univ. of South Australia (Australia)
Robert D. Short, Univ. of South Australia (Australia)
Hans J. Griesser, Univ. of South Australia (Australia)

Published in SPIE Proceedings Vol. 8204:
Smart Nano-Micro Materials and Devices
Saulius Juodkazis; Min Gu, Editor(s)

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