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Proceedings Paper

Laser based enhancement of susceptibility of bacteria to antibiotic
Author(s): Yana Reznick; Ehud Banin; Anat Lipovsky; Rachel Lubart; Zeev Zalevsky
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Paper Abstract

Our objective is to test the effect of pulsed (Q-switched) and continuous wave (CW) laser light at wavelength of 532nm on the viability of free-living stationary phase bacteria with and without gentamicin (an antibiotic) treatment. Free living stationary phase gram negative bacteria (Pseudomonas aeruginosa strain PAO1) was immersed in Luria Broth (LB) solution and exposed to Q-switched and CW lasers with and without the addition of the antibiotic gentamicin. Cell viability was determined at different time points. Laser treatment alone did not reduce cell viability compared to untreated control and the gentamicin treatment alone only resulted in a 0.5 log reduction in the viable count for P. aeruginosa. The combined laser and gentamicin treatment, however, resulted in a synergistic effect and viability was reduced by 8 log's for P. aeruginosa PAO1.

Paper Details

Date Published: 14 February 2012
PDF: 10 pages
Proc. SPIE 8224, Biophotonics and Immune Responses VII, 82240K (14 February 2012); doi: 10.1117/12.901520
Show Author Affiliations
Yana Reznick, Bar-Ilan Univ. (Israel)
Ehud Banin, Bar-Ilan Univ. (Israel)
Anat Lipovsky, Bar-Ilan Univ. (Israel)
Rachel Lubart, Bar-Ilan Univ. (Israel)
Zeev Zalevsky, Bar-Ilan Univ. (Israel)

Published in SPIE Proceedings Vol. 8224:
Biophotonics and Immune Responses VII
Wei R. Chen, Editor(s)

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