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Proceedings Paper

Mechanics of DNA: sequence dependent elasticity
Author(s): Krishnan Raghunathan; Yih-Fan Chen; Justin Blaty; Benjamin Arthur Juliar; Joshua Milstein; Jens-Christian Meiners
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Paper Abstract

We have used constant force axial optical tweezers to understand the subtle eects of sequence variations on the mechanical properties of DNA. Using designed sequences of DNA with nearly identical curvatures, but varied AT content, we have shown the persistence length to be highly dependent on the elasticity of DNA. The persistence length varies by almost thirty percent between sequences containing 61% AT and 45% AT. The biological implications of this can be substantial, as the need to bend DNA is involved in a host of regulatory schemes, ranging from nucleosome positioning to the formation of protein-mediated repressor and enhancer loops.

Paper Details

Date Published: 9 September 2011
PDF: 8 pages
Proc. SPIE 8097, Optical Trapping and Optical Micromanipulation VIII, 80970C (9 September 2011); doi: 10.1117/12.895297
Show Author Affiliations
Krishnan Raghunathan, Univ. of Michigan (United States)
Yih-Fan Chen, Univ. of Michigan (United States)
Justin Blaty, Univ. of Michigan (United States)
Benjamin Arthur Juliar, Univ. of Michigan (United States)
Joshua Milstein, Univ. of Michigan (United States)
Jens-Christian Meiners, Univ. of Michigan (United States)

Published in SPIE Proceedings Vol. 8097:
Optical Trapping and Optical Micromanipulation VIII
Kishan Dholakia; Gabriel C. Spalding, Editor(s)

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