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Proceedings Paper

Extracting the ridge set as a graph for quantification of actin filament images obtained by confocal laser scanning microscopy
Author(s): Harald Birkholz
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Paper Abstract

The progress in image acquisition technique provides life sciences with an abundance of data. Image analysis facilitates the assessment. The actin cytoskeleton plays a crucial role in understanding the behavior of osteoblastic cells on biomaterials. It can be visualized by confocal laser scanning microscopy. In these images it appears as a dense network of bright ridges which is so far only qualitatively assessed. For quantification there is a need for ridge detection techniques which provide a geometrical description of this graph-like feature. The state of the art methods do not cope with the systematical degradation of the brightness information. This paper presents the key part of a ridge tracking technique, which makes more efficient use of context information. Two random models confirm the accuracy and highlight the area for further improvement of the method.

Paper Details

Date Published: 12 July 2011
PDF: 8 pages
Proc. SPIE 8000, Tenth International Conference on Quality Control by Artificial Vision, 800006 (12 July 2011); doi: 10.1117/12.895269
Show Author Affiliations
Harald Birkholz, Univ. of Rostock (Germany)

Published in SPIE Proceedings Vol. 8000:
Tenth International Conference on Quality Control by Artificial Vision
Jean-Charles Pinoli; Johan Debayle; Yann Gavet; Frédéric Gruy; Claude Lambert, Editor(s)

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