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Proceedings Paper

Investigating the interaction forces between T cells and antigen-presenting cells using an optical trapping system
Author(s): Amanda J. Wright; Robert A. Benson; Richard W. Bowman; Graham M. Gibson; Miles J. Padgett; John M. Girkin; James Brewer; Paul Garside
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Paper Abstract

The interactions between T cells and antigen-presenting cells (APCs) are crucial in triggering a successful antigenspecific, adaptive immune response leading to protection against a particular pathogen or disease. At present very little is known about the magnitudes of the forces involved in these interactions. We present results showing for the first time that optical tweezers can be used to measure these cell-cell interaction forces. We were able to see a significant difference in the force distributions taken with and without antigen, as evidenced by a Mann-Whitney U-test. The T cells of interest were trapped directly and no exogenous beads were added to the sample. Interaction forces between T cells and APCs in the presence of specific antigen ranged from 0-6.5 pN, whereas, when the specific antigen was absent the interaction forces ranged from 0-1.5 pN. The accuracy of the system will be discussed in terms of how we tracked the position of the optically trapped cell and the methods we used to minimise cell roll.

Paper Details

Date Published: 9 September 2011
PDF: 10 pages
Proc. SPIE 8097, Optical Trapping and Optical Micromanipulation VIII, 80970I (9 September 2011); doi: 10.1117/12.895113
Show Author Affiliations
Amanda J. Wright, Univ. of Strathclyde (United Kingdom)
Robert A. Benson, Univ. of Glasgow (United Kingdom)
Richard W. Bowman, Univ. of Glasgow (United Kingdom)
Graham M. Gibson, Univ. of Glasgow (United Kingdom)
Miles J. Padgett, Univ. of Glasgow (United Kingdom)
John M. Girkin, Univ. of Strathclyde (United Kingdom)
Durham Univ. (United Kingdom)
James Brewer, Univ. of Glasgow (United Kingdom)
Paul Garside, Univ. of Glasgow (United Kingdom)

Published in SPIE Proceedings Vol. 8097:
Optical Trapping and Optical Micromanipulation VIII
Kishan Dholakia; Gabriel C. Spalding, Editor(s)

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